Serial intravascular staining reveals altered T cell trafficking in aged and SIV +rhesus macaques

Abstract Lymphocyte trafficking is required for lymphocyte development and maturation, immune surveillance response, immunological memory. While integral a functional system, it may also play role in dysfunction. Patients with HIV the elderly have dysfunction characterized by increased inflammation, activation, susceptibility to infections cancer. As implicated these processes, we hypothesized that cohorts altered kinetics, resulting immunosenescence. To determine relationship between kinetics dysfunction, performed serial intravascular staining (SIVS) healthy, aged, SIV-infected rhesus macaques. SIVS uses sequential infusions of fluorescent antibody label cells vivo, enabling us follow them as they migrate over time. In all animals, found naïve central memory T underwent more faster into tissues while effector terminal were likely remain circulation. SIV +and aged observed subsets tissues. A possible result spend less time lymphoid tissue therefore be initiate an response. Increased cytotoxic entering potentially increasing hallmark immunosenesence both +patients. Using evaluate +rhesus provides new insights crucial system. Supported Intramural Research Program Vaccine Center, National Institute Allergy Infectious Disease, Institutes Health